|
Regression equation, correlation coefficients, linearity ranges and limit of detection and quantitation for the markers of Gegen Tang granule |
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| Compounds |
Regression equation |
Linear range (μg/mL) |
R2 |
LOD (ng/mL) |
LOQ (ng/mL) |
Intra-day RSD% (n = 6) |
Inter-day RSD% (n = 3) |
|
|
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| Puerarin |
y = 40149x – 5483 |
1.70 – 69.70 |
0.9997 |
6.21 |
20.70 |
0.83 |
0.78 |
| Paeoniflorin |
y = 13018x + 3168 |
1.07 – 64.36 |
0.9996 |
19.64 |
65.46 |
0.99 |
0.45 |
| Cinnamic acid |
y = 68413x + 2472 |
0.15 – 5.81 |
0.9998 |
3.72 |
12.40 |
0.61 |
0.60 |
| Daidzain |
y = 44698x + 350 |
0.06 – 4.96 |
0.9997 |
5.58 |
18.60 |
0.33 |
1.83 |
| Glycyrrhizin |
y = 5695x – 3089 |
1.07 – 85.26 |
0.9998 |
45.47 |
151.56 |
0.10 |
1.70 |
| Ephedrine |
y = 21411x +1949 |
1.08 – 86.13 |
0.9998 |
3.82 |
12.73 |
0.52 |
1.55 |
| Pseudoephedrine |
y = 30269x – 41321 |
2.98 – 119.13 |
0.9996 |
2.71 |
9.03 |
0.37 |
0.41 |
|
All regression results are statistically significant (P < 0.05) | |||||||
Song et al. Chinese Medicine 2007 2:7 doi:10.1186/1749-8546-2-7 |
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