Table 2

Regression equation, correlation coefficients, linearity ranges and limit of detection and quantitation for the markers of Gegen Tang granule

Compounds
Regression equation
Linear range (μg/mL)
R2
LOD (ng/mL)
LOQ (ng/mL)
Intra-day RSD% (n = 6)
Inter-day RSD% (n = 3)

Puerarin
y = 40149x – 5483
1.70 – 69.70
0.9997
6.21
20.70
0.83
0.78
Paeoniflorin
y = 13018x + 3168
1.07 – 64.36
0.9996
19.64
65.46
0.99
0.45
Cinnamic acid
y = 68413x + 2472
0.15 – 5.81
0.9998
3.72
12.40
0.61
0.60
Daidzain
y = 44698x + 350
0.06 – 4.96
0.9997
5.58
18.60
0.33
1.83
Glycyrrhizin
y = 5695x – 3089
1.07 – 85.26
0.9998
45.47
151.56
0.10
1.70
Ephedrine
y = 21411x +1949
1.08 – 86.13
0.9998
3.82
12.73
0.52
1.55
Pseudoephedrine
y = 30269x – 41321
2.98 – 119.13
0.9996
2.71
9.03
0.37
0.41

All regression results are statistically significant (P < 0.05)

Song et al. Chinese Medicine 2007 2:7   doi:10.1186/1749-8546-2-7