Figure 4.

Osteogenic properties of FLL aqueous extract in rat MSCs. Dose effect of FLL aqueous extract on (A) alkaline phosphatase (ALP) activity and (B) extracellular matrix content in MSCs were determined under osteogenic induction on day 7 and day 14 respectively. Data are the mean ± SD (n = 3) from three independent experiments. Significant difference: * P < 0.05; ** P < 0.01; *** P < 0.001 for difference from respective baseline cultures without treatment. (C) Calcium deposition at different treatment concentrations (0, 50 and 100 μg/mL) was visualized by Alizarin Red S staining on day 14.