Research

Quality evaluation of mycelial Antrodia camphorata using high-performance liquid chromatography (HPLC) coupled with diode array detector and mass spectrometry (DAD-MS)

Sandy Shuo Zhao and Kelvin Sze-Yin Leung

Department of Chemistry, Hong Kong Baptist University, Kowloon, Hong Kong SAR, China

author email corresponding author email

Chinese Medicine 2010, 5:4doi:10.1186/1749-8546-5-4

Published:	29 January 2010

Abstract

Background

Antrodia camphorata (AC) is an important fungus native to Taiwanese forested regions. Scientific studies have demonstrated that extracts of AC possess a variety of pharmacological functions. This study aims to identify the full profile fingerprint of nucleosides and nucleobases in mycelial AC and to assess the quality of two commercial mycelial AC products.

Methods

High-performance liquid chromatography coupled with diode array detector and mass spectrometry was employed to identify the major components in mycelial AC. The chemical separation was carried out using a gradient program on a reverse phase Alltima C₁₈ AQ analytical column (250 × 4.6 mm, 5 μm) with the mobile phase consisting of deionized water and methanol.

Results

Ten nucleosides and nucleobases, two maleimide derivatives, and a sterol were identified as the major constituents in mycelial AC. These groups of chemical compounds constitute the first chromatographic fingerprint as an index for quality assessment of this medicinal fungus.

Conclusions

This study provides the first chromatographic fingerprint to assess the quality of mycelial AC.