Research
Cytotoxic and apoptotic effects of six herbal plants against the human hepatocarcinoma (HepG2) cell line
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* Corresponding author: Natthida Weerapreeyakul natthida@kku.ac.th
1 Graduate School, Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen 40002, Thailand
2 Center for Research and Development of Herbal Health Products, Division of Pharmaceutical Chemistry, Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen 40002, Thailand
3 Centre for Research and Development of Medical Diagnostic Laboratories (CMDL), Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen 40002, Thailand
Chinese Medicine 2011, 6:39 doi:10.1186/1749-8546-6-39
Published: 31 October 2011Additional files
Additional file 1:
HPLC chromatograms of the polyphenolic compounds and flavonoids. Column: HiQ-Sil C18W reversed-phase column; flow rate, 0.7 ml/min. The mobile phase consisted of 20% acetonitrile in 80% Milli-Q water, 0.1% H3PO4 detected at 213 nm (left) and 280 nm (right). (A) gallic acid; (B) chlorogenic acid; (C) catechin; (D) epicatechin; (E) caffeic acid; (F) vanillic acid; (G) vanillin; (H) coumaric acid; (I) ferulic acid; (J) quercetin; and (K) solvent front or DMSO.
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Additional file 2:
HPLC fingerprints of the crude extracts. Column: HiQ-Sil C18W reversed-phase column; flow rate, 0.7 ml per minute. The mobile phase consisted of 20% acetonitrile in 80% Milli-Q water, 0.1% H3PO4 detected at 213 nm (left) and 280 nm (right). (A) G. daltonii extract; (B) C. orientalis extract; (C) C. speciosum extract; (D) A. tatarinowii extract; (E) A. villosum extract; and (F) P. kesiya extract.
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